BiopestPeptidV1, Main, Exploration, bibRecord, 000777

Non-Covalent Associates of siRNAs and AuNPs Enveloped with Lipid Layer and Doped with Amphiphilic Peptide for Efficient siRNA Delivery.

Identifieur interne : 000777 ( Main/Exploration ); précédent : 000776; suivant : 000778

Non-Covalent Associates of siRNAs and AuNPs Enveloped with Lipid Layer and Doped with Amphiphilic Peptide for Efficient siRNA Delivery.

Auteurs : Julia Poletaeva [Russie] ; Ilya Dovydenko [Russie] ; Anna Epanchintseva [Russie] ; Kseniya Korchagina [Russie] ; Dmitrii Pyshnyi [Russie] ; Evgeny Apartsin [Russie] ; Elena Ryabchikova [Russie] ; Inna Pyshnaya [Russie]

Source :

International journal of molecular sciences [ 1422-0067 ] ; 2018.

RBID : pubmed:30029512

Descripteurs français

KwdFr :
- Concentration en ions d'hydrogène (MeSH), Extinction de l'expression des gènes (MeSH), Humains (MeSH), Lignée cellulaire (MeSH), Lipides (composition chimique), Liposomes (composition chimique), Nanoparticules métalliques (composition chimique), Nanoparticules métalliques (ultrastructure), Or (composition chimique), Peptides (composition chimique), Petit ARN interférent (métabolisme), Protéines à fluorescence verte (métabolisme), Techniques de transfert de gènes (MeSH), Tensioactifs (composition chimique).
MESH :
- composition chimique : Lipides, Liposomes, Nanoparticules métalliques, Or, Peptides, Tensioactifs.
- métabolisme : Petit ARN interférent, Protéines à fluorescence verte.
- ultrastructure : Concentration en ions d'hydrogène, Extinction de l'expression des gènes, Humains, Lignée cellulaire, Nanoparticules métalliques, Techniques de transfert de gènes.

English descriptors

KwdEn :
- Cell Line (MeSH), Gene Silencing (MeSH), Gene Transfer Techniques (MeSH), Gold (chemistry), Green Fluorescent Proteins (metabolism), Humans (MeSH), Hydrogen-Ion Concentration (MeSH), Lipids (chemistry), Liposomes (chemistry), Metal Nanoparticles (chemistry), Metal Nanoparticles (ultrastructure), Peptides (chemistry), RNA, Small Interfering (metabolism), Surface-Active Agents (chemistry).
MESH :
- chemical , chemistry : Gold, Lipids, Liposomes, Peptides, Surface-Active Agents.
- chemical , metabolism : Green Fluorescent Proteins, RNA, Small Interfering.
- chemistry : Metal Nanoparticles.
- ultrastructure : Metal Nanoparticles.
- Cell Line, Gene Silencing, Gene Transfer Techniques, Humans, Hydrogen-Ion Concentration.

Abstract

Elaboration of non-viral vehicles for delivery of therapeutic nucleic acids, in particular siRNA, into a cell is an actively growing field. Gold nanoparticles (AuNPs) occupy a noticeable place in these studies, and various nanoconstructions containing AuNPs are reported. We aimed our work to the rational design of AuNPs-based siRNA delivery vehicle with enhanced transfection efficiency. We optimized the obtaining of non-covalent siRNAs-AuNPs cores: ionic strength, temperature and reaction time were determined. Formation of cores was confirmed using gel electrophoresis. Stable associates were prepared, and then enveloped into a lipid layer composed of phosphatidylcholine, phosphatidylethanolamine and novel pH-sensitive lipidoid. The constructions were modified with [Str-(RL)₄G-NH₂] peptide (the resulting construction). All intermediate and resulting nanoconstructions were analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) to control their physico-chemical properties. To examine the biological effect of the delivery vehicle, green fluorescent protein (GFP)-expressing human embryonic kidney (HEK) Phoenix cells were incubated with the resulting construction containing anti-GFP siRNA, with the siRNA effect being studied by flow cytometry and confocal microscopy. Transfection of the cells with the resulting construction reduced the GFP fluorescence as efficiently as Lipofectamin 3000. Thus, siRNA vehicle based on non-covalently bound siRNA-AuNP core and enveloped into a lipid layer provides efficient delivery of siRNA into a cell followed by specific gene silencing.

DOI: 10.3390/ijms19072096
PubMed: 30029512
PubMed Central: PMC6073485

Affiliations:

Russie

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000748
to stream Main, to step Curation: 000748

Le document en format XML

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<term>Gene Silencing (MeSH)</term>
<term>Gene Transfer Techniques (MeSH)</term>
<term>Gold (chemistry)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Humans (MeSH)</term>
<term>Hydrogen-Ion Concentration (MeSH)</term>
<term>Lipids (chemistry)</term>
<term>Liposomes (chemistry)</term>
<term>Metal Nanoparticles (chemistry)</term>
<term>Metal Nanoparticles (ultrastructure)</term>
<term>Peptides (chemistry)</term>
<term>RNA, Small Interfering (metabolism)</term>
<term>Surface-Active Agents (chemistry)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Concentration en ions d'hydrogène (MeSH)</term>
<term>Extinction de l'expression des gènes (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Lignée cellulaire (MeSH)</term>
<term>Lipides (composition chimique)</term>
<term>Liposomes (composition chimique)</term>
<term>Nanoparticules métalliques (composition chimique)</term>
<term>Nanoparticules métalliques (ultrastructure)</term>
<term>Or (composition chimique)</term>
<term>Peptides (composition chimique)</term>
<term>Petit ARN interférent (métabolisme)</term>
<term>Protéines à fluorescence verte (métabolisme)</term>
<term>Techniques de transfert de gènes (MeSH)</term>
<term>Tensioactifs (composition chimique)</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Gold</term>
<term>Lipids</term>
<term>Liposomes</term>
<term>Peptides</term>
<term>Surface-Active Agents</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term>
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<term>Liposomes</term>
<term>Nanoparticules métalliques</term>
<term>Or</term>
<term>Peptides</term>
<term>Tensioactifs</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Petit ARN interférent</term>
<term>Protéines à fluorescence verte</term>
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<term>Gene Silencing</term>
<term>Gene Transfer Techniques</term>
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<term>Hydrogen-Ion Concentration</term>
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<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Concentration en ions d'hydrogène</term>
<term>Extinction de l'expression des gènes</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Nanoparticules métalliques</term>
<term>Techniques de transfert de gènes</term>
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<front><div type="abstract" xml:lang="en">Elaboration of non-viral vehicles for delivery of therapeutic nucleic acids, in particular siRNA, into a cell is an actively growing field. Gold nanoparticles (AuNPs) occupy a noticeable place in these studies, and various nanoconstructions containing AuNPs are reported. We aimed our work to the rational design of AuNPs-based siRNA delivery vehicle with enhanced transfection efficiency. We optimized the obtaining of non-covalent siRNAs-AuNPs cores: ionic strength, temperature and reaction time were determined. Formation of cores was confirmed using gel electrophoresis. Stable associates were prepared, and then enveloped into a lipid layer composed of phosphatidylcholine, phosphatidylethanolamine and novel pH-sensitive lipidoid. The constructions were modified with [Str-(RL)₄G-NH₂] peptide (the resulting construction). All intermediate and resulting nanoconstructions were analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) to control their physico-chemical properties. To examine the biological effect of the delivery vehicle, green fluorescent protein (GFP)-expressing human embryonic kidney (HEK) Phoenix cells were incubated with the resulting construction containing anti-GFP siRNA, with the siRNA effect being studied by flow cytometry and confocal microscopy. Transfection of the cells with the resulting construction reduced the GFP fluorescence as efficiently as Lipofectamin 3000. Thus, siRNA vehicle based on non-covalently bound siRNA-AuNP core and enveloped into a lipid layer provides efficient delivery of siRNA into a cell followed by specific gene silencing.</div>
</front>
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<Month>10</Month>
<Day>29</Day>
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<DateRevised><Year>2018</Year>
<Month>11</Month>
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<Issue>7</Issue>
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<Day>19</Day>
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<Title>International journal of molecular sciences</Title>
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<ArticleTitle>Non-Covalent Associates of siRNAs and AuNPs Enveloped with Lipid Layer and Doped with Amphiphilic Peptide for Efficient siRNA Delivery.</ArticleTitle>
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<Abstract><AbstractText>Elaboration of non-viral vehicles for delivery of therapeutic nucleic acids, in particular siRNA, into a cell is an actively growing field. Gold nanoparticles (AuNPs) occupy a noticeable place in these studies, and various nanoconstructions containing AuNPs are reported. We aimed our work to the rational design of AuNPs-based siRNA delivery vehicle with enhanced transfection efficiency. We optimized the obtaining of non-covalent siRNAs-AuNPs cores: ionic strength, temperature and reaction time were determined. Formation of cores was confirmed using gel electrophoresis. Stable associates were prepared, and then enveloped into a lipid layer composed of phosphatidylcholine, phosphatidylethanolamine and novel pH-sensitive lipidoid. The constructions were modified with [Str-(RL)₄G-NH₂] peptide (the resulting construction). All intermediate and resulting nanoconstructions were analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) to control their physico-chemical properties. To examine the biological effect of the delivery vehicle, green fluorescent protein (GFP)-expressing human embryonic kidney (HEK) Phoenix cells were incubated with the resulting construction containing anti-GFP siRNA, with the siRNA effect being studied by flow cytometry and confocal microscopy. Transfection of the cells with the resulting construction reduced the GFP fluorescence as efficiently as Lipofectamin 3000. Thus, siRNA vehicle based on non-covalently bound siRNA-AuNP core and enveloped into a lipid layer provides efficient delivery of siRNA into a cell followed by specific gene silencing.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Poletaeva</LastName>
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<AffiliationInfo><Affiliation>Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia. dovydenko.il@gmail.com.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Epanchintseva</LastName>
<ForeName>Anna</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia. annaepanch@gmail.com.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Korchagina</LastName>
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<AffiliationInfo><Affiliation>Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia. pyshnyi@niboch.nsc.ru.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Apartsin</LastName>
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<AffiliationInfo><Affiliation>Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia. eka@niboch.nsc.ru.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Ryabchikova</LastName>
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<Identifier Source="ORCID">0000-0003-4714-1524</Identifier>
<AffiliationInfo><Affiliation>Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia. lenryab@yandex.ru.</Affiliation>
</AffiliationInfo>
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<ForeName>Inna</ForeName>
<Initials>I</Initials>
<Identifier Source="ORCID">0000-0002-7559-2376</Identifier>
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	Serveur d'exploration sur les peptides biopesticides
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Serveur d'exploration sur les peptides biopesticides

Non-Covalent Associates of siRNAs and AuNPs Enveloped with Lipid Layer and Doped with Amphiphilic Peptide for Efficient siRNA Delivery.

Non-Covalent Associates of siRNAs and AuNPs Enveloped with Lipid Layer and Doped with Amphiphilic Peptide for Efficient siRNA Delivery.

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